久久高清超碰?V热热久久|久久久综合精品|国产精品日韩专区欧美专区|亚洲精品另类欧美|欧美精品AⅤ一区二区三区|绯色av一区二区三区亚洲人妻|久久久久人妻精品一区|亚洲中文久久精品无码软件

Cell Line Name：

EGFR L858R-T790M-C797S/BaF3

Host Cell:

Ba/F3

Application:

Anti-proliferation assay and PD assay

Freeze Medium:

90% FBS+10% DMSO

Complete Culture Medium:

RPMI-1640+10%FBS

Mycoplasma Status:

Negative

EGFR is widely recognized for its importance in cancer. Amplification and mutations have been shown to be driving events in many cancer types. Its role in non-small cell lung cancer, glioblastoma and basal-like breast cancers has spurred many research and drug development efforts. Tyrosine kinase inhibitors have shown efficacy in EGFR amplfied tumors, most notably gefitinib and erlotinib. Mutations in EGFR have been shown to confer resistance to these drugs, particularly the variant T790M, which has been functionally characterized as a resistance marker for both of these drugs. The later generation TKI's have seen some success in treating these resistant cases, and targeted sequencing of the EGFR locus has become a common practice in treatment of non-small cell lung cancer. Overproduction of ligands is another possible mechanism of activation of EGFR. ERBB ligands include EGF, TGF-a, AREG, EPG, BTC, HB-EGF, EPR and NRG1-4 (for detailed information please refer to the respective ligand section). In ligand-activated cancers, Cetuximab appears to be more effective than tyrosine-kinase inhibitors.

1. WB of EGFR-L858R/T790M/C797S expression

Figure 1. WB of EGFR Expression

Lane 1: Negative control

Lane 2: EGFR-WT

Lane 3: EGFR-L858R/T790M

Lane 4: EGFR-L858R/T790M/C797S

2. Sanger sequencing

Figure 2. Sanger Sequencing of EGFR-L858R/T790M/C797S

3. Anti-proliferation assay

Figure 3. Anti-proliferation assay of three reference compounds on the EGFR L858R-T790M-C797S/BaF3 Stable Cell Line